Translational_Unit
pGPD-eGFP-

Part:BBa_K3271028:Design

Designed by: Setti Belhouari   Group: iGEM19_uOttawa   (2019-10-16)


pGPD-eGFP-tCYC1 in pSB1K3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1179


Design Notes

The downstream region of the GPD promoter was modified slightly to eliminate illegal restriction sites.


Source

eGFP sequence from BBa_K895006 was used. GPD promoter and CYC1 terminators were obtained from the endogenous loci in S. cerevisiae.

References